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CELL STEM CELL: 1. 8. 2013

CELL STEM CELL: 1. 8. 2013

CELL STEM CELL: Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA

"The generation of human induced pluripotent stem cells (iPSCs) holds great promise for the development of regenerative medicine therapies to treat a wide range of human diseases. However, the generation of iPSCs in the absence of integrative DNA vectors remains problematic. Here, we report a simple, highly reproducible RNA-based iPSC generation approach that utilizes a single, synthetic self-replicating VEE-RF RNA replicon that expresses four reprogramming factors (OCT4, KLF4, and SOX2, with c-MYC or GLIS1) at consistent high levels prior to regulated RNA degradation. A single VEE-RF RNA transfection into newborn or adult human fibroblasts resulted in efficient generation of iPSCs with all the hallmarks of stem cells, including cell surface markers, global gene expression profiles, and in vivo pluripotency, to differentiate into all three germ layers. The VEE-RF RNA-based approach has broad applicability for the generation of iPSCs for ultimate use in human stem cell therapies in regenerative medicine."

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00259-2

 

CELL STEM CELL: Rewiring Immunity: Generating a Functional Thymus from hESC.  Are We There Yet?

"Two papers in this issue of Cell Stem Cell have made a significant advance in solving one of the great challenges of modern immunology-resurrecting thymus function through the induction of thymus epithelial cells (TECs) by directed differentiation of human embryonic stem cells (hESCs)."

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00323-8

 

CELL STEM CELL: Ethical and Legal Issues Arising in Research on Inducing Human Germ Cells from Pluripotent Stem Cells

"Derivation of eggs or sperm from pluripotent stem cells or direct reprogramming from somatic cells would have huge effects on assisted reproductive technology. Here we discuss important ethical, legal, and social issues that would be raised by the development of such female or male gametes for clinical use."

http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00313-5